In vitro activity of Acanthamoeba castellanii on human platelets and 1 erythrocytes
نویسندگان
چکیده
A.Mattana, 1* L. Alberti, 1 G. Delogu, 2 P.L. Fiori, 2 and P. Cappuccinelli 2 3 Department of Pharmacological Sciences 1 and Department of Biomedical Sciences, Division of 4 Experimental and Clinical Microbiology, 2 University of Sassari, Sassari, Italy 5 6 7 Corresponding Author: Dr. Antonella Mattana 8 9 Mailing address: Dipartimento di Scienze del Farmaco 10 Via Muroni 23/A 11 07100 Sassari -Italy12 13 14 Tel: +39-079-228744 / 228711 15 Fax: +39-079-228715 16 E-mail: [email protected] 17 18 19 20 21 22 23 24 25 26 AC CE PT ED Copyright © 2008, American Society for Microbiology and/or the Listed Authors/Institutions. All Rights Reserved. Infect. Immun. doi:10.1128/IAI.00202-08 IAI Accepts, published online ahead of print on 17 November 2008
منابع مشابه
In vitro activity of Acanthamoeba castellanii on human platelets and erythrocytes.
The effect of Acanthamoeba on human platelets and erythrocytes has not been fully elucidated. This paper reports that cell-free supernatants prepared from A. castellanii can activate human platelets, causing both a significant increase in the cytosolic free-calcium concentration and platelet aggregation. In addition, we demonstrated that platelet activation depends on the activity of ADP consti...
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A crucial requirement for establishing corneal infection by the extracellular protozoal parasite, Acanthamoeba, is the ability of the parasite to bind to the corneal surface. In a series of in vitro studies, we examined the ability of Acanthamoeba castellanii [corrected] to adhere, invade, and damage normal, intact corneas of 11 mammalian and one avian species. A. castellanii [corrected] (80-90...
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Acanthamoeba castellanii is member of free living amoeba that may cause painful sight-threatening keratitis and life threatening encephalitis which involves central nervous system. Treatments for both infections are problematic because of the amoebic cysts resistance to therapeutic agents. Here we evaluated in vitro strength of methanolic seed extract of Peganum harmala on Acanthamoeba cysts an...
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PURPOSE Acanthamoeba trophozoites express a mannose binding receptor that facilitates adhesion of trophozoites to mannosylated proteins on corneal epithelial cells. This study was undertaken to determine the role that mannose stimulation has in the amoeba's growth, secreted products, and ability to desquamate the corneal epithelium. METHODS Acanthamoeba castellanii trophozoites were grown in ...
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